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Identification of genes expressed in tumor-associated macrophages

Gottfried, Eva ; Faust, Stefan ; Fritsche, Jana ; Kunz-Schughart, Leoni A. ; Andreesen, Reinhard ; Miyake, Kensuke ; Kreutz, Marina


Most malignant tumors contain so-called tumor-associated macrophages (TAM) as a major component of their leukocytic infiltrate. To investigate the impact of the tumor microenvironment on activation and differentiation of macrophages, we established a 3-dimensional model system by culturing human monocytes within multicellular tumor spheroids. After 7 days, monocyte-derived TAM were isolated and ...


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