Krause, S. W., Rothe, G., Gnad, M., Reichle, A. and Andreesen, Reinhard
(2003)
Blood leukocyte subsets and cytokine profile after autologous peripheral blood stem cell transplantation.
Annals of hematology 82 (10), pp. 628-36.
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Abstract
High-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT) includes the risk of infectious complications due to neutropenia and therapy-induced immune deviation. In order to understand early immune recovery in this situation, we analyzed the distribution of cell subsets by flow cytometry and we measured cytokine production in a whole blood assay stimulated with ...
Abstract
High-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT) includes the risk of infectious complications due to neutropenia and therapy-induced immune deviation. In order to understand early immune recovery in this situation, we analyzed the distribution of cell subsets by flow cytometry and we measured cytokine production in a whole blood assay stimulated with lipopolysaccharide (LPS) in order to induce monocyte (MO) activation in 43 patients with solid tumors or lymphoma treated with two cycles of high-dose chemotherapy and PBSCT. Blood was collected at the following time points: before start of mobilization chemotherapy, before and after high-dose chemotherapy, and 10 and 30 days after PBSCT. In the lymphocyte compartment, we found a depletion of B cells and naive T cells and a transitory reduction of natural killer (NK) cells, whereas MO and neutrophils recovered rapidly. However, during early recovery, HLA-DR expression on MO and the percentage of CD16(+) MO was considerably reduced. Production of proinflammatory cytokines interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)-alpha upon LPS stimulation was severely impaired directly after chemotherapy and unexpectedly remained low during early recovery of myeloid cells, whereas production of IL-1RA was enhanced, indicating a shift of immune competent cells to an anti-inflammatory or anergic state early after PBSCT.
Export bibliographical data
| Item type: | Article |
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| Date: | 2003 |
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| Institutions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
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| Identification Number: | | Value | Type |
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| 12961030 | PubMed ID | | 10.1007/s00277-003-0716-z | DOI |
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| Classification: | | Notation | Type |
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| Antineoplastic Combined Chemotherapy Protocols/therapeutic use | MESH | | Cytokines/immunology | MESH | | Female | MESH | | Flow Cytometry | MESH | | Hematopoietic Stem Cell Transplantation/methods | MESH | | Humans | MESH | | Immunophenotyping | MESH | | Lipopolysaccharides/pharmacology | MESH | | Lymphocyte Subsets/immunology | MESH | | Male | MESH | | Monocytes/immunology | MESH | | Neutrophil Activation/immunology | MESH | | Transplantation Conditioning | MESH | | Transplantation, Autologous | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Yes |
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| Item ID: | 14416 |
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