Item type: | Article | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Journal or Publication Title: | Annals of hematology | ||||||||||||||||||||||||||||||
Publisher: | SPRINGER | ||||||||||||||||||||||||||||||
Place of Publication: | NEW YORK | ||||||||||||||||||||||||||||||
Volume: | 82 | ||||||||||||||||||||||||||||||
Number of Issue or Book Chapter: | 10 | ||||||||||||||||||||||||||||||
Page Range: | pp. 628-36 | ||||||||||||||||||||||||||||||
Date: | 2003 | ||||||||||||||||||||||||||||||
Institutions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin | ||||||||||||||||||||||||||||||
Identification Number: |
| ||||||||||||||||||||||||||||||
Classification: |
| ||||||||||||||||||||||||||||||
Keywords: | BONE-MARROW TRANSPLANTATION; COLONY-STIMULATING FACTOR; HIGH-DOSE CHEMOTHERAPY; CD8(+) T-CELLS; INTENSIVE CHEMOTHERAPY; LYMPHOCYTE SUBSETS; CANCER-PATIENTS; BREAST-CANCER; MONOCYTES; RELEASE; hematopoietic stem cell transplantation; antineoplastic drug therapy; leukocyte subsets; cytokines; hematopoietic recovery | ||||||||||||||||||||||||||||||
Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine | ||||||||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||||||||
Refereed: | Yes, this version has been refereed | ||||||||||||||||||||||||||||||
Created at the University of Regensburg: | Yes | ||||||||||||||||||||||||||||||
Item ID: | 14416 |
Abstract
High-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT) includes the risk of infectious complications due to neutropenia and therapy-induced immune deviation. In order to understand early immune recovery in this situation, we analyzed the distribution of cell subsets by flow cytometry and we measured cytokine production in a whole blood assay stimulated with ...

Abstract
High-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT) includes the risk of infectious complications due to neutropenia and therapy-induced immune deviation. In order to understand early immune recovery in this situation, we analyzed the distribution of cell subsets by flow cytometry and we measured cytokine production in a whole blood assay stimulated with lipopolysaccharide (LPS) in order to induce monocyte (MO) activation in 43 patients with solid tumors or lymphoma treated with two cycles of high-dose chemotherapy and PBSCT. Blood was collected at the following time points: before start of mobilization chemotherapy, before and after high-dose chemotherapy, and 10 and 30 days after PBSCT. In the lymphocyte compartment, we found a depletion of B cells and naive T cells and a transitory reduction of natural killer (NK) cells, whereas MO and neutrophils recovered rapidly. However, during early recovery, HLA-DR expression on MO and the percentage of CD16(+) MO was considerably reduced. Production of proinflammatory cytokines interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)-alpha upon LPS stimulation was severely impaired directly after chemotherapy and unexpectedly remained low during early recovery of myeloid cells, whereas production of IL-1RA was enhanced, indicating a shift of immune competent cells to an anti-inflammatory or anergic state early after PBSCT.
Metadata last modified: 29 Sep 2021 07:37