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Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice

Ewing, Patricia ; Wilke, Andrea ; Brockhoff, Gero ; Andreesen, Reinhard ; Eissner, Günther ; Holler, Ernst ; Gerbitz, Armin


The isolation of primary endothelial cells from murine tissues has long been a challenge and remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and enzymatic digestion, adherent ...


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