Go to content
UR Home

Isolation and transplantation of allogeneic pulmonary endothelium derived from GFP transgenic mice

Ewing, Patricia ; Wilke, Andrea ; Brockhoff, Gero ; Andreesen, Reinhard ; Eissner, Günther ; Holler, Ernst ; Gerbitz, Armin



Abstract

The isolation of primary endothelial cells from murine tissues has long been a challenge and remains a difficult task. Using GFP transgenic C57/BL6 mice as donors, we describe a reliable method to isolate pulmonary endothelial cells by flow cytometry after staining with DiI-Ac-low density lipoprotein (LDL). After mechanical dissociation of murine lung tissue and enzymatic digestion, adherent ...

plus


Owner only: item control page
  1. Homepage UR

University Library

Publication Server

Contact:

Publishing: oa@ur.de

Dissertations: dissertationen@ur.de

Research data: daten@ur.de

Contact persons