Rehli, Michael, Sulzbacher, Sabine, Pape, Sabine, Ravasi, Timothy, Wells, Christine A., Heinz, Sven, Söllner, Liane, El Chartouni, Carol, Krause, Stefan W., Steingrimsson, Eirikur, Hume, David A. and Andreesen, Reinhard
(2005)
Transcription factor Tfec contributes to the IL-4-inducible expression of a small group of genes in mouse macrophages including the granulocyte colony-stimulating factor receptor.
Journal of immunology (Baltimore, Md. : 1950) 174 (11), pp. 7111-22.
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Abstract
Expression of the mouse transcription factor EC (Tfec) is restricted to the myeloid compartment, suggesting a function for Tfec in the development or function of these cells. However, mice lacking Tfec develop normally, indicating a redundant role for Tfec in myeloid cell development. We now report that Tfec is specifically induced in bone marrow-derived macrophages upon stimulation with the Th2 ...
Abstract
Expression of the mouse transcription factor EC (Tfec) is restricted to the myeloid compartment, suggesting a function for Tfec in the development or function of these cells. However, mice lacking Tfec develop normally, indicating a redundant role for Tfec in myeloid cell development. We now report that Tfec is specifically induced in bone marrow-derived macrophages upon stimulation with the Th2 cytokines, IL-4 and IL-13, or LPS. LPS induced a rapid and transient up-regulation of Tfec mRNA expression and promoter activity, which was dependent on a functional NF-kappaB site. IL-4, however, induced a rapid, but long-lasting, increase in Tfec mRNA, which, in contrast to LPS stimulation, also resulted in detectable levels of Tfec protein. IL-4-induced transcription of Tfec was absent in macrophages lacking Stat6, and its promoter depended on two functional Stat6-binding sites. A global comparison of IL-4-induced genes in both wild-type and Tfec mutant macrophages revealed a surprisingly mild phenotype with only a few genes affected by Tfec deficiency. These included the G-CSFR (Csf3r) gene that was strongly up-regulated by IL-4 in wild-type macrophages and, to a lesser extent, in Tfec mutant macrophages. Our study also provides a general definition of the transcriptome in alternatively activated mouse macrophages and identifies a large number of novel genes characterizing this cell type.
Export bibliographical data
| Item type: | Article |
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| Date: | 2005 |
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| Institutions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
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| Identification Number: | | Value | Type |
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| 15908341 | PubMed ID |
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| Classification: | | Notation | Type |
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| Animals | MESH | | Base Sequence | MESH | | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors | MESH | | Bone Marrow Cells/metabolism | MESH | | Cell Line | MESH | | Cells, Cultured | MESH | | Gene Expression Profiling/methods | MESH | | Helix-Loop-Helix Motifs/immunology | MESH | | Immunophenotyping | MESH | | Interleukin-4/physiology | MESH | | Lipopolysaccharides/pharmacology | MESH | | Macrophages/metabolism | MESH | | Mice | MESH | | Mice, Inbred BALB C | MESH | | Mice, Knockout | MESH | | Molecular Sequence Data | MESH | | Oligonucleotide Array Sequence Analysis/methods | MESH | | Receptors, Granulocyte Colony-Stimulating Factor/genetics | MESH | | STAT6 Transcription Factor | MESH | | Signal Transduction/immunology | MESH | | Trans-Activators/physiology | MESH | | Transcription Factors/physiology | MESH | | Up-Regulation/immunology | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Yes, this version has been refereed |
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| Created at the University of Regensburg: | Yes |
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| Item ID: | 14451 |
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