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Rapid and sensitive detection of CpG-methylation using methyl-binding (MB)-PCR

Gebhard, Claudia ; Schwarzfischer, Lucia ; Pham, Thu Hang ; Andreesen, Reinhard ; Mackensen, Andreas ; Rehli, Michael



Abstract

Methylation of CpG islands is associated with transcriptional repression and, in cancer, leads to the abnormal silencing of tumor suppressor genes. We have developed a novel technique for detecting CpG-methylated DNA termed methyl-binding (MB)-PCR. This technique utilizes a recombinant protein with high affinity for CpG-methylated DNA that is coated onto the walls of a PCR vessel and selectively ...

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