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Extraction of cells from paraffin-embedded tissue sections for single-cell DNA cytophotometry.

Mikuz, G. ; Hofstaedter, Ferdinand ; Delgado, R.



Abstract

A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. Tissue fragments from paraffin blocks were deparaffinized in xylene, rehydrated and refixed in a formalin solution and incubated in a solution of 0.5 pepsin in 0.25% hydrochloric acid. After filtration through a 70 micron mesh and centrifugation, the cells were ...

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