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Isolation, culture and characterization of human pancreatic duct cells

Trautmann, B. ; Schlitt, Hans-Jürgen ; Hahn, E. G. ; Löhr, M.
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Date of publication of this fulltext: 10 May 2010 12:13


Abstract

To establish a suitable control for pancreatic tumor cell lines, we have isolated and cultured primary human pancreatic duct cells from transplant donors. Duct cells were isolated by dissecting the main pancreatic duct and first-degree branches and enzymatic digestion. Aggregates of cells were cultured for 1 up to 5 weeks and monitored for changes in morphology and growth by phase contrast ...

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