Responsiveness for mouse IgG2b antibodies to the human alpha/beta T-cell receptor/CD3 complex: evidence for genetic determination and low grade antibody cross-linking not mediated by known Fc gamma receptors

DOI zum Zitieren dieses Dokuments:

10.5283/epub.14714 Copy

Schlitt, Hans-Jürgen ; Schwinzer, R. ; Wonigeit, K.

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Veröffentlichungsdatum dieses Volltextes: 10 Mai 2010 12:12

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Details

Dokumentenart:	Artikel
Titel eines Journals oder einer Zeitschrift:	Scandinavian journal of immunology
Verlag:	Wiley-Blackwell
Band:	37
Nummer des Zeitschriftenheftes oder des Kapitels:	3
Seitenbereich:	S. 329-336
Datum:	März 1993
Institutionen:	Medizin > Lehrstuhl für Chirurgie
Identifikationsnummer:	Wert Typ 8441919 PubMed-ID 10.1111/j.1365-3083.1993.tb02561.x DOI
Klassifikation:	Notation Art Animals MESH Antibodies, Monoclonal/immunology MESH Antigen-Antibody Reactions MESH Antigen-Presenting Cells/immunology MESH Antigens, CD3/immunology MESH Humans MESH Immunoglobulin G/immunology MESH Immunoglobulin Isotypes/immunology MESH Mice MESH Pedigree MESH Receptors, Antigen, T-Cell, alpha-beta/immunology MESH Receptors, Fc/immunology MESH
Dewey-Dezimal-Klassifikation:	600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin
Status:	Veröffentlicht
Begutachtet:	Unbekannt / Keine Angabe
An der Universität Regensburg entstanden:	Unbekannt / Keine Angabe
Dokumenten-ID:	14714

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Zusammenfassung

Mouse IgG2a and IgG3 antibodies directed to the human T-cell receptor/CD3 complex stimulate peripheral blood mononuclear cells in almost all individuals. By contrast, responder and non-responder individuals exist for the stimulatory effect of mouse IgG1 and IgG2b antibodies. Whereas responsiveness to IgG1 antibodies is rather frequent (60-70%) and is known to be determined by an Fc gamma RII ...

Zusammenfassung

Mouse IgG2a and IgG3 antibodies directed to the human T-cell receptor/CD3 complex stimulate peripheral blood mononuclear cells in almost all individuals. By contrast, responder and non-responder individuals exist for the stimulatory effect of mouse IgG1 and IgG2b antibodies. Whereas responsiveness to IgG1 antibodies is rather frequent (60-70%) and is known to be determined by an Fc gamma RII polymorphism on the accessory cells, little is known about the underlying factors of the rare (6%) IgG2b responsiveness. In this study it is shown that (1) IgG2b responsiveness is genetically determined with a dominant pattern of inheritance; (2) IgG2b responsiveness is determined by a radioresistant feature of responder accessory cells which can be substituted by artificial cross-linking, but not by IL-1 beta or IL-2; (3) stimulation of peripheral blood mononuclear cells of an IgG2b responder by IgG2b antibodies requires rather high antibody concentration compared with stimulation by IgG2a antibodies; (4) the stimulation leads to proliferation and IL-2 receptor expression, but no measurable IL-2 production; (5) antibody binding to known Fc gamma receptors (Fc gamma RI, Fc gamma RII, Fc gamma RIII) is not involved in the stimulatory effect of the IgG2b antibodies in responders. These results demonstrate that responsiveness to IgG2b antibodies against the TcR/CD3 complex depends on a genetically determined feature of accessory cells that is not identical with any of the known Fc gamma receptors. Most likely, the stimulatory effect observed in IgG2b responders is explained by a low grade cross-linking of the antibody by a not yet identified polymorphic structure on the surface of accessory cells.

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