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A heterologous in vitro coculture system to study interaction between human bladder cancer cells and fibroblasts

Schuster, U. ; Büttner, R. ; Hofstädter, Ferdinand ; Knüchel, R.


Three-dimensional multicellular spheroids of two fibroblast cell lines (WI-38 and N1) and two differently differentiated bladder carcinoma cell lines (RT4 and J82) were used for cocultures of multicellular tumor spheroids with multicellular spheroids of fibroblasts. The aim of the study was the establishment and characterization of a standardized three-dimensional model for studies of tumor ...


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