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Multicellular bladder tumor spheroids in coculture with human endothelial cell monolayers

Knüchel, R. ; Hofstädter, Ferdinand ; Feichtinger, J. ; Recktenwald, A. ; Franke, R. P. ; Hollweg, H. ; Rübben, H. ; Rammal, E. ; Jakse, G.


Multicellular tumor spheroids (MCTS) are avascular spherical tumors grown systematically out of single cells in vitro. The coculture of MCTS of the human bladder carcinoma cell line J 82 with confluent human endothelial cell layers serves as a model to study cell-cell interactions as an important feature in hematogenous tumor spread. Basic information is obtained by phase contrast microscopy and ...


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