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Indocyanine green: intracellular uptake and phototherapeutic effects in vitro

Fickweiler, S. ; Szeimies, R. M. ; Bäumler, W. ; Steinbach, P. ; Karrer, S. ; Goetz, A. E. ; Abels, C. ; Hofstädter, Ferdinand ; Landthaler, M.


Indocyanine green (ICG; absorption peak in human plasma 805 nm) was investigated for ICG-mediated phototherapy in vitro. The cellular uptake of ICG (1 microM-50 microM) into HaCaT keratinocytes after an incubation period of 24 h increased up to an intracellular ICG concentration of 12.1 +/- 1.3 nmol per 10(6) cells. To examine dose dependent phototoxic effects in vitro, keratinocytes were ...


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