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Multiple mutation analyses in single tumor cells with improved whole genome amplification

Dietmaier, W. ; Hartmann, A. ; Wallinger, S. ; Heinmöller, E. ; Kerner, T. ; Endl, E. ; Jauch, K. W. ; Hofstädter, Ferdinand ; Rüschoff, J.



Abstract

Combining whole genome amplification (WGA) methods with novel laser-based microdissection techniques has made it possible to exploit recent progress in molecular knowledge of cancer development and progression. However, WGA of one or a few cells has not yet been optimized and systematically evaluated for samples routinely processed in tumor pathology. We therefore studied the value of established ...

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