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MethyQESD, a robust and fast method for quantitative methylation analyses in HNPCC diagnostics using formalin-fixed and paraffin-embedded tissue samples

Bettstetter, Marcus ; Dechant, Stefan ; Ruemmele, Petra ; Vogel, Corinna ; Kurz, Katrin ; Morak, Monika ; Keller, Gisela ; Holinski-Feder, Elke ; Hofstaedter, Ferdinand ; Dietmaier, Wolfgang



Abstract

Promoter hypermethylation occurs in various tumors and leads to silencing of tumor-relevant genes. Thus, promoter methylation analysis (MA) has been established as an important tool in cancer research and diagnostics. Here we present MethyQESD (methylation-quantification of endonuclease-resistant DNA) as a fast, easy, precise and reliable method for quantitative MA without the need of ...

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