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The use of 3-D cultures for high-throughput screening: the multicellular spheroid model

Kunz-Schughart, Leoni A. ; Freyer, James P. ; Hofstaedter, Ferdinand ; Ebner, Reinhard



Abstract

Over the past few years, establishment and adaptation of cell-based assays for drug development and testing has become an important topic in high-throughput screening (HTS). Most new assays are designed to rapidly detect specific cellular effects reflecting action at various targets. However, although more complex than cell-free biochemical test systems, HTS assays using monolayer or suspension ...

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