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Cryopreservation of human endothelial cells for vascular tissue engineering.

Lehle, Karla ; Hoenicka, Markus ; Jacobs, Volker R. ; Schmid, Franz X. ; Birnbaum, Dietrich E.


To investigate the influence of cryopreservation on endothelial cell growth, morphology, and function human umbilical vein endothelial cells (HUVECs) were frozen following a standard protocol. Cell suspensions were exposed to 10% dimethyl sulfoxide in a high-potassium solution, cooled to -80 degrees C at 1 degrees C/min and stored in liquid nitrogen for 7-36 days. Samples were thawed in a 37 ...


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