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Removing Water Artefacts from 2D Protein NMR Spectra using GEVD with Congruent Matrix Pencils

Stadlthanner, K. ; Tomé, A. M. ; Theis, Fabian J. ; Gronwald, W. ; Kalbitzer, Hans Robert ; Lang, Elmar



Abstract

Multidimensional H nmr spectra of biomolecules dissolved in light water are contaminated by an intense water artefact. We discuss the application of the generalized eigenvalue decomposition (GEVD) method using a matrix pencil to explore the time structure of the signals in order to separate out the water artefacts. Simulated as well as experimental 2D NOESY spectra of proteins are studied. Results are compared to those obtained with the FastICA algorithm.


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