Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly

URN to cite this document:: urn:nbn:de:bvb:355-epub-159562
DOI to cite this document:: 10.5283/epub.15956

Harvath, L. ; Falk, Werner ; Leonard, E.J.

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Date of publication of this fulltext: 20 Jul 2010 05:06

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Abstract

A neutrophil chemotaxis assay was developd which permits rapid, quantitative assessment of migration across a membrane filter. The critical factor in the assay was the use of a 10 microns thick polycarbonate membrane without the usual polyvinylpyrrolidone coating. Migrated neutrophils remain adherent to the uncoated membrane, whereas 20-50% fall off polyvinylpyrrolidone-coated membranes. A major ...