URN zum Zitieren dieses Dokuments: urn:nbn:de:bvb:355-epub-159649
Falk, Werner und Leonard, E.J.
(1982)
Chemotaxis of purified human monocytes in vitro: lack of accessory cell requirement.
Infection and immunity 36 (2), S. 591-597.
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Zusammenfassung
This study was undertaken to determine whether cell cooperation, either among monocytes or between monocytes and lymphocytes, is a prerequisite for monocyte chemotactic responsiveness. We compared Ficoll-Hypaque-separated mononuclear cells and a preparation of 99% pure monocytes obtained by chemotaxis in a newly designed separation chamber. Monocytes of both preparations migrated to ...
Zusammenfassung
This study was undertaken to determine whether cell cooperation, either among monocytes or between monocytes and lymphocytes, is a prerequisite for monocyte chemotactic responsiveness. We compared Ficoll-Hypaque-separated mononuclear cells and a preparation of 99% pure monocytes obtained by chemotaxis in a newly designed separation chamber. Monocytes of both preparations migrated to chemoattractants without a lag phase, and no further increase in migrated cells was observed after 70 min. The cell dose-response was linear for both preparations over a wide range of cell concentrations in the cell input well of the chemotaxis chamber, suggesting that no monocyte-monocyte interaction was required. Since only 20 to 60% of the monocytes purified by chemotaxis migrated a second time, the possibility of a requirement for an accessory cell was tested. The addition to purified monocytes of several different mononuclear cell preparations comprising lymphocytes or nonmigrating monocytes had no effect on monocyte migration. These experiments show that normal human blood monocytes in vitro do not require stimuli from other cells to respond to chemoattractants. Their behavior is profoundly different from that of mouse peritoneal macrophages, which exhibit a time lag in vitro before migration toward an attractant and become more responsive with either increasing cell concentration or addition of purified lymphocytes.
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| Dokumentenart: | Artikel |
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| Datum: | 1982 |
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| Institutionen: | Medizin > Lehrstuhl für Innere Medizin I |
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| Identifikationsnummer: | |
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| Klassifikation: | | Notation | Art |
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| Cell Communication | MESH | | Cell Count | MESH | | Cell Separation | MESH | | Chemotaxis, Leukocyte | MESH | | Complement C5 | MESH | | Complement C5a | MESH | | Humans | MESH | | Kinetics | MESH | | Lymphocytes/physiology | MESH | | Monocytes/physiology | MESH | | N-Formylmethionine/analogs & derivatives | MESH | | N-Formylmethionine Leucyl-Phenylalanine | MESH | | Oligopeptides | MESH |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
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| Eingebracht am: | 20 Jul 2010 09:20 |
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| Zuletzt geändert: | 08 Mrz 2017 08:27 |
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| Dokumenten-ID: | 15964 |
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