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Observation of Mg²⁺.ATP and uncomplexed ATP in slow exchange by ³¹P-NMR at high magnetic fields

Sontheimer, G. M. ; Kuhn, W. ; Kalbitzer, Hans Robert


The 31P-NMR lines of the beta-phosphate groups in Mg2+.ATP and in metalfree ATP can be observed separately up to 280 K at 8.5 T and up to 285 K at 11.7 T. At 274 K and 8.5 T the beta-phosphorous resonances are in slow exchange at pH values above pH 5, the gamma-phosphorous resonances are in slow exchange only near pH 6, but in fast exchange at low and high pH-values. The fast exchange condition ...


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