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Stereochemistry and lifetime of the GTP hydrolysis intermediate at the active site of elongation factor Tu from Bacillus stearothermophilus as inferred from the ¹⁷O-⁵⁵Mn superhyperfine interaction

Kalbitzer, Hans Robert ; Feuerstein, J. ; Goody, R. S. ; Wittinghofer, A.



Abstract

Electron paramagnetic resonance spectroscopy has been used to obtain information on the structure and stability of the products of GTP cleavage at the active site of elongation factor Tu (EF-Tu) from Bacillus stearothermophilus. Using stereospecifically labelled (Sp)-(Rp)-[beta-17O]GTP (prepared by modification of a previously published procedure which is now also suitable for guanine ...

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