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Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product

Wolber, V., Rensland, H., Brandmeier, B., Sagemann, M., Hoffmann, R., Kalbitzer, Hans Robert and Wittinghofer, A. (1992) Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product. European journal of biochemistry: EJB (= The FEBS journal) 205 (3), pp. 1115-1121.

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Abstract

The human immunodeficiency virus 1 (HIV-1) nef gene encoded by the HIV-1 isolate lymphadenopathy-associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple two-column purification scheme has been developed allowing isolation of the recombinant protein without using denaturing ...

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Item type:Article
Date:1992
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer
Identification Number:
ValueType
1533585PubMed ID
Classification:
NotationType
Adenine Nucleotides/metabolismMESH
Adenosine Triphosphatases/metabolismMESH
Base SequenceMESH
Chromatography, High Pressure LiquidMESH
Circular DichroismMESH
Electrophoresis, Polyacrylamide GelMESH
Escherichia coli/geneticsMESH
GTP Phosphohydrolases/metabolismMESH
GTP-Binding Proteins/metabolismMESH
Gene ExpressionMESH
Gene Products, nef/metabolismMESH
Genes, BacterialMESH
Guanine Nucleotides/metabolismMESH
HIV-1/metabolismMESH
Molecular Sequence DataMESH
PhosphorylationMESH
PlasmidsMESH
Promoter Regions, GeneticMESH
Recombinant Proteins/metabolismMESH
Spectrophotometry, UltravioletMESH
nef Gene Products, Human Immunodeficiency VirusMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:16465
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