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Expression, purification and biochemical characterisation of the human immunodeficiency virus 1 nef gene product

Wolber, V. ; Rensland, H. ; Brandmeier, B. ; Sagemann, M. ; Hoffmann, R. ; Kalbitzer, Hans Robert ; Wittinghofer, A.



Abstract

The human immunodeficiency virus 1 (HIV-1) nef gene encoded by the HIV-1 isolate lymphadenopathy-associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple two-column purification scheme has been developed allowing isolation of the recombinant protein without using denaturing ...

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