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A new high sensitivity ¹⁹F probe for labeling cysteine groups of proteins

Kalbitzer, Hans Robert ; Rohr, G. ; Nowak, E. ; Goody, R. S. ; Kuhn, W. ; Zimmermann, H.



Abstract

A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40 microM solution) can be detected easily in a single scan at 470 MHz.


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