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Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1

Kienzle, N. ; Freund, J. ; Kalbitzer, Hans Robert ; Müller-Lantzsch, N.


The nef genes, derived from two different human immunodeficiency-virus-type-1 (HIV-1) strains, were expressed in procaryotic cells (Escherichia coli) and in eucaryotic cells (insect cells infected with nef-containing baculovirus). The oligomerization of recombinant Nef protein was studied by NMR spectroscopy and immunoblotting under various experimental conditions. 1H-NMR spectroscopy shows that ...


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