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Chemical phosphorylation of the peptides GGXA (X = S; T; Y): an evaluation of different chemical approaches

Hoffmann, R. ; Wachs, W. O. ; Berger, R. G. ; Kalbitzer, Hans Robert ; Waidelich, D. ; Bayer, E. ; Wagner-Redeker, W. ; Zeppezauer, M.


An evaluation was made of the two methods most commonly used for phosphorylation of hydroxyamino acids in peptides, i.e. the tetrazole-catalysed phosphitylation by di-tert-butyl-N,N-diethylphosphoramidite followed by oxidation and the phosphorylation by dibenzylphosphochloridate. As model system the sequence GGXA (X = S, T, Y) was used which represents a random-coil sequence avoiding the ...


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