Overcoming the problems associated with poor spectra quality of the protein kinase Byr2 using residual dipolar couplings

Gronwald, W., Brunner, E., Huber, F., Wenzler, M., Herrmann, C. und Kalbitzer, Hans Robert (2001) Overcoming the problems associated with poor spectra quality of the protein kinase Byr2 using residual dipolar couplings. Protein science 10 (6), S. 1260-1263.

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Zusammenfassung

For the Ras-binding domain of the protein kinase Byr2, only a limited number of NOE contacts could be initially assigned unambiguously, as the quality of the NOESY spectra was too poor. However, the use of residual (1)H-(15)N dipolar couplings in the beginning of the structure determination process allows to overcome this problem. We used a three-step recipe for this procedure. A previously ...

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