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NMR-spectroscopic mapping of an engineered cavity in the I14A mutant of HPr from Staphylococcus carnosus using xenon

Gröger, Christian ; Möglich, Andreas ; Pons, Miguel ; Koch, Brigitte ; Hengstenberg, Wolfgang ; Kalbitzer, Hans Robert ; Brunner, Eike


The interaction between the histidine-containing phosphocarrier protein HPr and xenon atoms in solution is studied in the present paper. Wild-type HPr as well as the exchange mutant I14A have been studied. Specific binding of xenon into an engineered cavity created via the exchange of amino acid residue I14 by alanine could be shown using 1H-15N heteronuclear single-quantum coherence (HSQC) ...


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