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High-resolution structure of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus aureus and characterization of its interaction with the bifunctional HPr kinase/phosphorylase

Maurer, Till ; Meier, Sebastian ; Kachel, Norman ; Munte, Claudia Elisabeth ; Hasenbein, Sonja ; Koch, Brigitte ; Hengstenberg, Wolfgang ; Kalbitzer, Hans Robert



Abstract

A high-resolution structure of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus aureus was obtained by heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopy on the basis of 1,766 structural restraints. Twenty-three hydrogen bonds in HPr could be directly detected by polarization transfer from the amide nitrogen to the carbonyl carbon involved in the ...

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