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Relative Quantification of mRNA Levels in Jurkat T Cells with RT-Real Time-PCR (RT-rt-PCR): New Possibilities for the Screening of Anti-Inflammatory and Cytotoxic Compounds

Gertsch, Juerg ; Guettinger, Martin ; Sticher, Otto ; Heilmann, Jörg



Abstract

The objective of this study was quantification of the pro-inflammatory action of mitogens on mRNA levels of growth-related genes, transcription factors, and cytokines in T cells as markers for the screening of compds. with immunomodulatory, anti-inflammatory or cytotoxic potential. A reverse transcription-real time-polymerase chain reaction assay with TaqMan probes was developed. Jurkat T cells ...

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