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Relative Quantification of mRNA Levels in Jurkat T Cells with RT-Real Time-PCR (RT-rt-PCR): New Possibilities for the Screening of Anti-Inflammatory and Cytotoxic Compounds
Gertsch, Juerg, Guettinger, Martin, Sticher, Otto and Heilmann, Jörg (2002) Relative Quantification of mRNA Levels in Jurkat T Cells with RT-Real Time-PCR (RT-rt-PCR): New Possibilities for the Screening of Anti-Inflammatory and Cytotoxic Compounds. Pharmaceutical research 19 (8), pp. 1236-1243.Date of publication of this fulltext: 18 Oct 2010 11:01
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| Item type | Article | ||||
| Journal or Publication Title | Pharmaceutical research | ||||
| Publisher: | Springer | ||||
|---|---|---|---|---|---|
| Volume: | 19 | ||||
| Number of Issue or Book Chapter: | 8 | ||||
| Page Range: | pp. 1236-1243 | ||||
| Date | 2002 | ||||
| Additional Information (public) | CAN 138:265054 1-1 Pharmacology 83869-56-1 (GM-CSF) Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mRNA, response; relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.); 16561-29-8 (Phorbol 12-myristate 13-acetate) Role: ARG (Analytical reagent use), ANST (Analytical study), USES (Uses) (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.); 404-86-4 (Capsaicin); 548-04-9 (Hypericin); 7295-85-4 ((+-)-Catechin); 59865-13-3 (Cyclosporin A) Role: ANT (Analyte), PAC (Pharmacological activity), THU (Therapeutic use), ANST (Analytical study), BIOL (Biological study), USES (Uses) (validation of method; relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) | ||||
| Institutions | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) | ||||
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| Keywords | Cyclins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (D1, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Transcription factors Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (IkB-alpha (NF-kB inhibitor alpha ), mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Animal cell line (JURKAT relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Transcription factors Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (NFAT2 (nuclear factor of activated T-cell, 2), mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) PCR (RT-PCR (reverse transcription-PCR), real-time relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Fas antigen Interleukin 2 Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Proteins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (p65, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Anti-inflammatory agents Cytotoxic agents Drug screening Human (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) mRNA Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti- Actins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (beta -, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Interferons Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (gamma , mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) antiinflammatory cytotoxic agent screening mRNA real time RT PCR | ||||
| Dewey Decimal Classification | 500 Science > 570 Life sciences 500 Science > 540 Chemistry & allied sciences | ||||
| Status | Published | ||||
| Refereed | Yes, this version has been refereed | ||||
| Created at the University of Regensburg | No | ||||
| Item ID | 17202 |
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