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Relative Quantification of mRNA Levels in Jurkat T Cells with RT-Real Time-PCR (RT-rt-PCR): New Possibilities for the Screening of Anti-Inflammatory and Cytotoxic Compounds

Gertsch, Juerg, Guettinger, Martin, Sticher, Otto and Heilmann, Jörg (2002) Relative Quantification of mRNA Levels in Jurkat T Cells with RT-Real Time-PCR (RT-rt-PCR): New Possibilities for the Screening of Anti-Inflammatory and Cytotoxic Compounds. Pharmaceutical research 19 (8), pp. 1236-1243.

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Abstract

The objective of this study was quantification of the pro-inflammatory action of mitogens on mRNA levels of growth-related genes, transcription factors, and cytokines in T cells as markers for the screening of compds. with immunomodulatory, anti-inflammatory or cytotoxic potential. A reverse transcription-real time-polymerase chain reaction assay with TaqMan probes was developed. Jurkat T cells ...

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Item type:Article
Date:2002
Additional Information (public):CAN 138:265054 1-1 Pharmacology 83869-56-1 (GM-CSF) Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mRNA, response; relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.); 16561-29-8 (Phorbol 12-myristate 13-acetate) Role: ARG (Analytical reagent use), ANST (Analytical study), USES (Uses) (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.); 404-86-4 (Capsaicin); 548-04-9 (Hypericin); 7295-85-4 ((+-)-Catechin); 59865-13-3 (Cyclosporin A) Role: ANT (Analyte), PAC (Pharmacological activity), THU (Therapeutic use), ANST (Analytical study), BIOL (Biological study), USES (Uses) (validation of method; relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.)
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann)
Identification Number:
ValueType
2002:635334Other
Keywords:Cyclins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (D1, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Transcription factors Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (IkB-alpha (NF-kB inhibitor alpha ), mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Animal cell line (JURKAT relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Transcription factors Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (NFAT2 (nuclear factor of activated T-cell, 2), mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) PCR (RT-PCR (reverse transcription-PCR), real-time relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Fas antigen Interleukin 2 Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Proteins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (p65, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Anti-inflammatory agents Cytotoxic agents Drug screening Human (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) mRNA Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti- Actins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (beta -, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Interferons Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (gamma , mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) antiinflammatory cytotoxic agent screening mRNA real time RT PCR
Dewey Decimal Classification:500 Science > 570 Life sciences
500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Item ID:17202
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