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DNA Staining in Agarose Gels with ZN2+-Cyclen-Pyrene

Schmidt, Florian, Schmidt, Jennifer, Riechers, Alexander, Haase, Susann, Bosserhoff, Anja-Katrin, Heilmann, Jörg and Konig, Burkhard (2010) DNA Staining in Agarose Gels with ZN2+-Cyclen-Pyrene. Nucleosides, Nucleotides & Nucleic Acids 29 (10), pp. 748-759.

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Abstract

A pyrene-labeled Zn2+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amts. as low as 10 ng and ...

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Item type:Article
Date:2010
Additional Information (public):9 Biochemical Methods
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann)
Identification Number:
ValueType
2010:1254098Other
Dewey Decimal Classification:500 Science > 570 Life sciences
500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:17256
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