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DNA Staining in Agarose Gels with ZN2+-Cyclen-Pyrene

Schmidt, Florian ; Schmidt, Jennifer ; Riechers, Alexander ; Haase, Susann ; Bosserhoff, Anja-Katrin ; Heilmann, Jörg ; Konig, Burkhard



Abstract

A pyrene-labeled Zn2+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amts. as low as 10 ng and ...

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