Cell-wall-bound lytic activity in Chlorella fusca: function and characterization of an endo-mannanase

URN to cite this document:: urn:nbn:de:bvb:355-epub-175190
DOI to cite this document:: 10.5283/epub.17519

Loos, Eckhard ; Meindl, Doris

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Date of publication of this fulltext: 26 Oct 2010 14:22

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Abstract

A cell-wall-degrading activity was solubilized from young cells and from mother cell walls of Chlorella fusca by treatment with LiCl. The cytoplasmic enzyme hexokinase was not detectable in these extracts. The LiCl-solubilized activity increased in the cell cycle parallel to the release of autospores. The enzyme was purified on a chromatofocusing column followed by gel filtration. Sodium dodecyl ...