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A simple method of delayed processing of tumor tissue in a soft agar clonogenic assay

Krischke, W., Hartmann, R., Schneider, M. and Schönenberger, Helmut (1988) A simple method of delayed processing of tumor tissue in a soft agar clonogenic assay. Journal of cancer research and clinical oncology 114 (2), pp. 170-176.

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Sarcoma 180 tumor tissue from C57 mice was processed in a soft agar clonogenic assay immediately after removal from the animal and after various methods of storage. The sensitivity to the antineoplastic drug cis-platinum was not affected by different storage methods. The highest yield of colony forming cells per 100 mg tumor tissue was 2.3 X 10(4) cells following immediate processing of fresh ...


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Item type:Article
Additional Information (public):GERMANY, WEST: Germany, Federal Republic of 15663-27-1 (Cisplatin); 9002-18-0 (Agar)
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Alumni or Retired Professors > Prof. Schönenberger
Projects:SFB 234
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Keywords:Agar Animals Cell Survival Cisplatin: PD, pharmacology *Colony-Forming Units Assay Freezing Mice Tissue Preservation *Tumor Cells, Cultured: DE, drug effects *Tumor Stem Cell Assay
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:17670
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