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A simple method of delayed processing of tumor tissue in a soft agar clonogenic assay

Krischke, W. ; Hartmann, R. ; Schneider, M. ; Schönenberger, Helmut



Abstract

Sarcoma 180 tumor tissue from C57 mice was processed in a soft agar clonogenic assay immediately after removal from the animal and after various methods of storage. The sensitivity to the antineoplastic drug cis-platinum was not affected by different storage methods. The highest yield of colony forming cells per 100 mg tumor tissue was 2.3 X 10(4) cells following immediate processing of fresh ...

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