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Impact of normothermic perfusion and protein supplementation on human endothelial cell function during organ preservation

Puehler, Thomas ; Gleich, Otto ; Schopka, Simon ; Rupprecht, Leopold ; Hirt, Stephan ; Schmid, Christof ; Lehle, Karla


BACKGROUND: Hypothermia-induced changes in endothelial cell (EC) morphology and function after organ storage may influence the initial outcome and development of transplant-associated coronary artery disease. METHODS: Human saphenous vein ECs were incubated with saline (NaCl), University of Wisconsin (UW), and histidine-tryptophan-ketoglutarate (HTK) solution, with and without protein additives, ...


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