Hillenkamp, Jost, Hussain, Ali A., Jackson, Timothy L., Cunningham, Joanna R. und Marshall, John
(2006)
Effect of taurine and apical potassium concentration on electrophysiologic parameters of bovine retinal pigment epithelium.
Experimental eye research 82 (2), S. 258-264.
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Andere URL zum Volltext: http://dx.doi.org/10.1016/j.exer.2005.06.020
Zusammenfassung
The purpose of this study was to assess the effect of taurine and apical potassium concentration modelling in vivo light evoked changes on the transepithelial potential (TEP) and the transepithelial resistance (TER) of isolated bovine retinal pigment epithelium (RPE). Isolated specimens of bovine non-tapetal RPE-Bruch's-choroid (RPE-BC) were mounted in modified Ussing chambers. The apical and the ...
Zusammenfassung
The purpose of this study was to assess the effect of taurine and apical potassium concentration modelling in vivo light evoked changes on the transepithelial potential (TEP) and the transepithelial resistance (TER) of isolated bovine retinal pigment epithelium (RPE). Isolated specimens of bovine non-tapetal RPE-Bruch's-choroid (RPE-BC) were mounted in modified Ussing chambers. The apical and the basolateral side of the preparations were exposed to 10 mm and 10 microm concentrations of taurine in Krebs' medium with either 6.04 or 2.2 mm potassium in the apical compartment. TEP and TER were recorded over 140 min. TEP and TER decreased with exposure to taurine over the course of 1 hr followed by a stabilisation. The degree of this response did not depend on the concentration of taurine but was more pronounced when taurine was added to the apical compartment. Lowering apical potassium from 6.04 to 2.2 mm further pronounced the decrease of TEP and TER. The data show that light-induced release of taurine from the outer retina and light-induced decrease of the potassium concentration in the subretinal space synergistically lead to a temporary decrease in TEP and TER. Thereby, taurine uptake into the RPE is reduced probably by a reduction of the activity of the electrogenic Na+/taurine co-transporter of the apical RPE cell membrane. The findings suggest a mechanism whereby the sustained presence of taurine in the interphotoreceptor matrix following exposure to light may protect photoreceptor outer segments from light-induced oxidative stress.
Bibliographische Daten exportieren
| Dokumentenart: | Artikel |
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| Datum: | Februar 2006 |
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| Institutionen: | Medizin > Lehrstuhl für Augenheilkunde |
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| Identifikationsnummer: | | Wert | Typ |
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| 16102750 | PubMed-ID | | 10.1016/j.exer.2005.06.020 | DOI |
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| Stichwörter / Keywords: | retinal pigment epithelium; taurine; electrophysiology; apical potassium |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
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| Eingebracht am: | 30 Mär 2007 |
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| Zuletzt geändert: | 04 Jun 2018 14:23 |
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| Dokumenten-ID: | 1930 |
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