Multiparameter immunofluorescence on paraffin-embedded tissue sections

DOI to cite this document:: 10.5283/epub.1936

Bataille, Frauke ; Troppmann, Sabine ; Klebl, Frank ; Rogler, Gerhard

; Stoelcker, Benjamin ; Hofstaedter, Ferdinand ; Bosserhoff, Anja-Katrin ; Rümmele, Petra

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Date of publication of this fulltext: 05 Aug 2009 13:34

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Abstract

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin ...