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A new weakly basic amino-reactive fluorescent label for use in isoelectric focusing and chip electrophoresis

Schulze, Philipp, Link, Martin, Schulze, Marcel, Thürmann, Sebastian, Wolfbeis, Otto S. and Belder, Detlev (2010) A new weakly basic amino-reactive fluorescent label for use in isoelectric focusing and chip electrophoresis. Electrophoresis 31 (16), pp. 2749-2753.

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Date of publication of this fulltext: 18 Mar 2011 06:45

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Abstract

In this study, we present a novel amino-reactive fluorescence marker (referred to as UR-431), which is well suited for electrophoretic techniques. A main feature of this marker is its weakly basic behavior when conjugated to analytes. Labeled primary amines exhibit a positive net charge and accordingly a cathodic mobility below a pH of 2.4. The label features a pH-independent fluorescence ...

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Item type:Article
Date:August 2010
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1002/elps.201000007DOI
Keywords:CIEF; Fluorescence marker; MCE; Luminescence detection; Protein labeling
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:19961
Owner only: item control page

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