A new weakly basic amino-reactive fluorescent label for use in isoelectric focusing and chip electrophoresis

DOI to cite this document:: 10.5283/epub.19961

Schulze, Philipp ; Link, Martin ; Schulze, Marcel ; Thürmann, Sebastian ; Wolfbeis, Otto S. ; Belder, Detlev

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Date of publication of this fulltext: 18 Mar 2011 06:45

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Abstract

In this study, we present a novel amino-reactive fluorescence marker (referred to as UR-431), which is well suited for electrophoretic techniques. A main feature of this marker is its weakly basic behavior when conjugated to analytes. Labeled primary amines exhibit a positive net charge and accordingly a cathodic mobility below a pH of 2.4. The label features a pH-independent fluorescence ...