Determination of biogenic amines by capillary electrophoresis using a chameleon type of fluorescent stain

DOI to cite this document:: 10.5283/epub.20004

Steiner, Mark-Steven ; Meier, Robert J. ; Spangler, Christian ; Dürkop, Axel

; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Mar 2011 07:01

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Abstract

A method was developed for the determination of biogenic amines (BAs) via micellar electrokinetic chromatography along with laser induced fluorescence detection using the amino-reactive chameleon stain Py-1. A labeling protocol was established for seven primary BAs by optimizing the reaction conditions in terms of the amount of reagents, reaction temperature, reaction time and solvent. ...