Highly resolved dose–response functions for drug-modulated bacteria cultivation obtained by fluorometric and photometric flow-through sensing in microsegmented flow

Funfak, Anette ; Hartung, Regina ; Cao, Jialan ; Martin, Karin ; Wiesmüller, Karl-Heinz ; Wolfbeis, Otto S. ; Köhler, J. Michael

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Date of publication of this fulltext: 18 Mar 2011 06:58

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Details

Item type:	Article
Journal or Publication Title:	Sensors and Actuators B: Chemical
Publisher:	Elsevier
Volume:	142
Number of Issue or Book Chapter:	1
Page Range:	pp. 66-72
Date:	12 October 2009
Institutions:	Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:	Value Type 10.1016/j.snb.2009.07.017 DOI
Keywords:	Micro fluid segments; Droplet-based flow; Antibiotics; Drug detection; Dose–response functions; On-line pH sensing; Cell density monitoring
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	20006

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Abstract

The application of the micro fluid segment technique for the investigation of highly resolved dose–response relationship at the physiological parameters segment–internal pH and growth of the cell culture is introduced. The method was used to study the concentration-dependent response of an Escherichia coli culture for the effectors 2,4-dinitrophenol (DNP) and an antibacterial peptide amide ...

Abstract

The application of the micro fluid segment technique for the investigation of highly resolved dose–response relationship at the physiological parameters segment–internal pH and growth of the cell culture is introduced. The method was used to study the concentration-dependent response of an Escherichia coli culture for the effectors 2,4-dinitrophenol (DNP) and an antibacterial peptide amide (KKVVFKVKFK-NH2). Large sequences with up to 250 micro fluid segments containing gradually varying concentrations of the effectors were generated using a PTFE micro fluidic arrangement, including a 7-port-manifold and PC-controlled syringe pumps. The response of the cell culture was characterized by a double sensor system allowing for simultaneous read out of metabolism-related changes as well as changes in cell density. A twin arrangement of a micro flow-through photometer and a micro flow-through fluorometer based on LED devices in connection with the application of pH-sensitive polymer sensor particles was applied. This experimental setup allows a detailed determination of drug-related changes in fluorescence intensity by the E. coli culture and the polymer particles as a function of time by tracking changes in pH and cell density. The application of the segmented flow technique for multi-parameter drug screenings provides new insights into the biological answer of bacteria cultures cultivated at the nanoliter scale.

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