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Probing the Activity of Matrix Metalloproteinase II with a Sequentially Click-Labeled Silica Nanoparticle FRET Probe

Achatz, Daniela E. ; Mező, Gábor ; Kele, Peter ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 06:59


Abstract

Selective double click labeling: The determination of nanomolar concentrations of matrix metalloproteinase II is made possible through the use of a nanoprobe that consists of fluorescently doped silica nanoparticles to which a FRET-based enzyme substrate has been conjugated through sequential click reactions.


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