Monitoring cell cultivation in microfluidic segments by optical pH sensing with a micro flow-through fluorometer using dye-doped polymer particles

DOI to cite this document:: 10.5283/epub.20047

Funfak, Anette ; Cao, Jialan ; Wolfbeis, Otto S.

; Martin, Karin ; Köhler, J. Michael

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Date of publication of this fulltext: 18 Mar 2011 07:15

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Abstract

Polymer microparticles containing an immobilized pH-sensitive dye are used for determination of pH inside microfluidic segments. The particles possess a hydrophilic surface in order to get a homogenous distribution inside the aqueous phase of microfluidic segments. The dye is coupled to the polymer matrix by a covalent bond. The pH can be determined by the read-out of fluorescence intensity. In ...