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Monitoring cell cultivation in microfluidic segments by optical pH sensing with a micro flow-through fluorometer using dye-doped polymer particles

Funfak, Anette, Cao, Jialan, Wolfbeis, Otto S., Martin, Karin and Köhler, J. Michael (2009) Monitoring cell cultivation in microfluidic segments by optical pH sensing with a micro flow-through fluorometer using dye-doped polymer particles. Microchimica Acta 164 (3-4), pp. 279-286.

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Abstract

Polymer microparticles containing an immobilized pH-sensitive dye are used for determination of pH inside microfluidic segments. The particles possess a hydrophilic surface in order to get a homogenous distribution inside the aqueous phase of microfluidic segments. The dye is coupled to the polymer matrix by a covalent bond. The pH can be determined by the read-out of fluorescence intensity. In ...

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Item type:Article
Date:2009
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1007/s00604-008-0096-0DOI
Keywords:Microfluidic segments - Droplet-based flow - Sensor particles - Flow-through measurement - Microfluorometry
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20047
Owner only: item control page

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