Monitoring cell cultivation in microfluidic segments by optical pH sensing with a micro flow-through fluorometer using dye-doped polymer particles

Funfak, Anette ; Cao, Jialan ; Wolfbeis, Otto S. ; Martin, Karin ; Köhler, J. Michael

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Date of publication of this fulltext: 18 Mar 2011 07:15

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Details

Item type:	Article
Journal or Publication Title:	Microchimica Acta
Publisher:	Springer Verlag
Volume:	164
Number of Issue or Book Chapter:	3-4
Page Range:	pp. 279-286
Date:	2009
Institutions:	Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:	Value Type 10.1007/s00604-008-0096-0 DOI
Keywords:	Microfluidic segments - Droplet-based flow - Sensor particles - Flow-through measurement - Microfluorometry
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	20047

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Abstract

Polymer microparticles containing an immobilized pH-sensitive dye are used for determination of pH inside microfluidic segments. The particles possess a hydrophilic surface in order to get a homogenous distribution inside the aqueous phase of microfluidic segments. The dye is coupled to the polymer matrix by a covalent bond. The pH can be determined by the read-out of fluorescence intensity. In ...

Abstract

Polymer microparticles containing an immobilized pH-sensitive dye are used for determination of pH inside microfluidic segments. The particles possess a hydrophilic surface in order to get a homogenous distribution inside the aqueous phase of microfluidic segments. The dye is coupled to the polymer matrix by a covalent bond. The pH can be determined by the read-out of fluorescence intensity. In contrast to dissolved indicator dyes, the chemical interference of the sensor particles with the surrounding solution is negligible. So, the particle-based sensing can easily be applied to the determination of pH changes during the cultivation of cells inside the microfluidic segments. The typical change of pH during cell cultivation can be used for monitoring the kinetic of cell cultivation inside single volumes in the nanoliter range, so that information about the metabolic activity of the organism can be obtained. An LED-based miniaturized flow-through fluorometer was developed to determine the fluorescence directly inside microtubes of an inner diameter of 0.5 mm. It allows measurement frequencies up to 60 Hz and is suited for characterization of fast moving large sequences of microfluidic segments.

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Metadata last modified: 03 Jun 2018 05:36

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