SDS-PAGE of Proteins Using a Chameleon-Type of Fluorescent Prestain

DOI to cite this document:: 10.5283/epub.20054

Meier, Robert J. ; Steiner, Mark-Steven ; Dürkop, Axel

; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Mar 2011 07:28

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Abstract

A new prestaining method for protein SDS-PAGE was developed using the fluorogenic amino-reactive label Py-1. This resulted in one of the fastest, most sensitive, and environmentally friendly protocols available. It is mainly due to the unique optical properties of Py-1, which is blue and virtually nonfluorescent but turns to red and becomes much more strongly fluorescent once it is conjugated to ...