Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH

DOI to cite this document:: 10.5283/epub.20056

Kocincová, Anna S. ; Nagl, Stefan ; Arain, Sarina ; Krause, Christian ; Borisov, Sergey M. ; Arnold, Matthias ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Mar 2011 07:30

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Abstract

Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a ...