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Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH

Kocincová, Anna S. ; Nagl, Stefan ; Arain, Sarina ; Krause, Christian ; Borisov, Sergey M. ; Arnold, Matthias ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 07:30


Abstract

Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a ...

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