Probing DNA Hybridization in Homogeneous Solution and at Interfaces via Measurement of the Intrinsic Fluorescence Decay Time of a Single Label

DOI to cite this document:: 10.5283/epub.20057

Höfelschweiger, Bianca K. ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Mar 2011 07:32

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Abstract

The hybridization of DNA oligomers including molecular beacons can be detected by measurement of either the decay time or the intensity of a single fluorescent label attached to the end of the respective oligonucleotide. The method works both in solution and solid phase and can distinguish between fully complementary and mismatch sequences as demonstrated for a 15-mer oligonucleotide and a 25-mer ...