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Modified Dual Lifetime Referencing Method for Simultaneous Optical Determination and Sensing of Two Analytes

Borisov, Sergey M. ; Neurauter, Gerhard ; Schröder, Claudia R. ; Klimant, Ingo ; Wolfbeis, Otto S.


Simultaneous fluorometric sensing of two analytes becomes possible using a modified dual lifetime referencing (m-DLR) method. In this scheme, two luminescent indicators are needed that have overlapping absorption and emission spectra but largely different decay times. They are excited by a single light source, and both emissions are measured simultaneously. In the frequency domain m-DLR method, ...


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