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Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays

Arain, Sarina ; John, Gernot T. ; Krause, Christian ; Gerlach, Jochen ; Wolfbeis, Otto S. ; Klimant, Ingo
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Date of publication of this fulltext: 04 Apr 2011 10:29


Abstract

This paper describes properties of microplates (MTPs) with integrated, fluorescence-based sensors for pH and oxygen, and their application for enzyme screening and monitoring of bacterial respiratory activity. Thin, hydrophilic sensing films consisting of an analyte-sensitive indicator and a reference fluorophore, are deposited on the bottom of the MTP. This allows for calibration-free ...

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