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Microtiter plate assay for phosphate using a europium–tetracycline complex as a sensitive luminescent probe

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Dürkop, Axel ; Turel, Matejka ; Lobnik, Aleksandra ; Wolfbeis, Otto S.
Date of publication of this fulltext: 04 Apr 2011 10:31


A new luminescent europium probe is presented for the determination of phosphate (P) in microtiter plate format. The assay is based on the quenching of the luminescence of the europium–tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The ...


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