Microtiter plate assay for phosphate using a europium–tetracycline complex as a sensitive luminescent probe

URN to cite this document:: urn:nbn:de:bvb:355-epub-202981
DOI to cite this document:: 10.5283/epub.20298

Dürkop, Axel

; Turel, Matejka

; Lobnik, Aleksandra ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 04 Apr 2011 10:31

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Abstract

A new luminescent europium probe is presented for the determination of phosphate (P) in microliter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 mu mol/L. The probe is excited at 400 nm and displays a large Stokes' shift of 210 nm. The emission maximum is located at 616 nm. ...