Novel type of general protein assay using a chromogenic and fluorogenic amine-reactive probe

DOI to cite this document:: 10.5283/epub.20354

Höfelschweiger, Bianca K. ; Dürkop, Axel

; Wolfbeis, Otto S.

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Date of publication of this fulltext: 04 Apr 2011 10:34

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Abstract

We report on a new and simple one-reagent method for general protein assay. it makes use of one of two new reactive labeling reagents presented here (and referred to as pyrylium [Py] labels). These can be applied for both photometric and fluorometric protein assays at near neutral pHs at room temperature. The Py labels undergo a large spectral change on conjugation to the amino group of proteins ...