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Novel type of general protein assay using a chromogenic and fluorogenic amine-reactive probe

Höfelschweiger, Bianca K. ; Dürkop, Axel ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 04 Apr 2011 10:34


Abstract

We report on a new and simple one-reagent method for general protein assay. It makes use of one of two new reactive labeling reagents presented here (and referred to as pyrylium [Py] labels). These can be applied for both photometric and fluorometric protein assays at near neutral pHs at room temperature. The Py labels undergo a large spectral change on conjugation to the amino group of proteins ...

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