Determination of picomolar concentrations of proteins using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection

DOI to cite this document:: 10.5283/epub.20358

Craig, Douglas B. ; Wetzl, Bianca K. ; Dürkop, Axel

; Wolfbeis, Otto S.

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Date of publication of this fulltext: 04 Apr 2011 10:39

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Abstract

Py-1 and Py-6 are novel amino-reactive fluorescent reagents. The names given to them reflect that they consist of a pyrylium group attached to small aromatic moieties. Upon reaction with a primary amine there is a large spectral shift in the reagent, rendering them effectively fluorogenic. In this study, these reagents were used to label a test protein, (human serum albumin), and the sample was ...