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Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe

Wu, Meng ; Lin, Zhihong ; Schäferling, Michael ; Dürkop, Axel ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 04 Apr 2011 10:46


Abstract

A method for optical imaging of the activity of glucose oxidase (GOx) using a fluorescent europium(III) tetracycline probe for hydrogen peroxide is presented. A decay time in the microsecond range and the large Stokes shift of 210 nm of the probe facilitate intensity-based, time-resolved, and decay-time-based imaging of glucose oxidase. Four methods for imaging the activity of GOx were compared, ...

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