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Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe

Wu, Meng, Lin, Zhihong, Schäferling, Michael, Dürkop, Axel and Wolfbeis, Otto S. (2005) Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe. Analytical Biochemistry 340 (1), pp. 66-73.

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Abstract

A method for optical imaging of the activity of glucose oxidase (GOx) using a fluorescent europium(III) tetracycline probe for hydrogen peroxide is presented. A decay time in the microsecond range and the large Stokes shift of 210 nm of the probe facilitate intensity-based, time-resolved, and decay-time-based imaging of glucose oxidase. Four methods for imaging the activity of GOx were compared, ...

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Item type:Article
Date:1 May 2005
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1016/j.ab.2005.01.050DOI
Keywords:Glucose oxidase; Enzyme imaging; Fluorescent probe; Time-resolved imaging; Enzyme activity assay
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20364
Owner only: item control page

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