Double-Wavelength Technique for Surface Plasmon Resonance Measurements: Basic Concept and Applications for Single Sensors and Two-Dimensional Sensor Arrays

DOI to cite this document:: 10.5283/epub.20365

Zybin, Alexander ; Grunwald, Christian ; Mirsky, Vladimir M.

; Kuhlmann, Jürgen ; Wolfbeis, Otto S.

; Niemax, Kay

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Date of publication of this fulltext: 04 Apr 2011 10:47

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Abstract

A new technique for on-line monitoring of analyte binding to sensor surfaces by surface plasmon resonance (SPR) detection is described. It is based on differential measurements using two wavelengths provided by two diode lasers. The technique is as simple and robust as the conventional SPR detection measuring the reflected radiation at fixed incidence angle, but it has the advantage of being ...