Double-Wavelength Technique for Surface Plasmon Resonance Measurements: Basic Concept and Applications for Single Sensors and Two-Dimensional Sensor Arrays

DOI to cite this document:

10.5283/epub.20365 Copy

Zybin, Alexander ; Grunwald, Christian ; Mirsky, Vladimir M. ; Kuhlmann, Jürgen ; Wolfbeis, Otto S. ; Niemax, Kay

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Date of publication of this fulltext: 04 Apr 2011 10:47

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Details

Item type:	Article
Journal or Publication Title:	Analytical Chemistry (including News & Features)
Publisher:	American Chemical Society
Volume:	77
Number of Issue or Book Chapter:	8
Page Range:	pp. 2393-2399
Date:	15 March 2005
Institutions:	Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:	Value Type 10.1021/ac048156v DOI
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	20365

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Abstract

A new technique for on-line monitoring of analyte binding to sensor surfaces by surface plasmon resonance (SPR) detection is described. It is based on differential measurements using two wavelengths provided by two diode lasers. The technique is as simple and robust as the conventional SPR detection measuring the reflected radiation at fixed incidence angle, but it has the advantage of being ...

Abstract

A new technique for on-line monitoring of analyte binding to sensor surfaces by surface plasmon resonance (SPR) detection is described. It is based on differential measurements using two wavelengths provided by two diode lasers. The technique is as simple and robust as the conventional SPR detection measuring the reflected radiation at fixed incidence angle, but it has the advantage of being nonsensitive to variations of the resonance width and providing essentially higher signal/noise ratios. The paper presents the first four channel prototype system for parallel 2D-monitoring at four different spots. One channel is always used as a reference to compensate temperature fluctuations and nonspecific adsorptions. Calibration with sucrose solutions revealed an absolute sensitivity of Δn 5 × 10-6. The new technique is tested with a biotin−streptavidin binding and with hybridization/denaturation of DNA. Biotin binding to a streptavidin monolayer is detected with a signal/noise ratio of about 5, which demonstrates the high potential of the new technique for applications in drug discovery. Applications to gene analysis are tested with short oligonucleotides of the sequences used for genotyping human hepatitis C viruses. A selective response to complementary oligonucleotides is observed. The high reproducibility in subsequent cycles of hybridization/denaturation (by formamide or by heating) points out potential applications of the technique in medical diagnostics, food industry, genomics, and proteomics too.

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Metadata last modified: 26 Nov 2020 07:10

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