A New Fluorescence Resonance Energy Transfer Pair and Its Application to Oligonucleotide Labeling and Fluorescence Resonance Energy Transfer Hybridization Studies

DOI to cite this document:: 10.5283/epub.20366

Gruber, Michaela ; Wetzl, Bianca ; Oswald, Bernhard ; Enderlein, Jörg ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 04 Apr 2011 10:48

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Abstract

We describe two new fluorescence resonance energy transfer (FRET) compatible labels, their covalent linkage to oligonucleotides, and their use as donor and acceptor, respectively, in FRET hybridization studies. The dyes belong to the cyanine dyes, and water solubility is imparted by a phosphonate which represents a new solubilizing group in DNA labels. They were linked to amino-modified synthetic ...